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Vitamin C

Vitamin C

Description

Vitamin C (ascorbic acid) is water soluble, white crystalline material stable in dry form. Much ascorbic acid is lost in cooking, but the loss can be reduced by cooking quickly in small amounts of water, or steaming foods with the pot covered tightly. Preservation is also accomplished via quick freezing.

Vitamin C has many functions: it can function as a coenzyme or as a cofactor in the body. It appears to be necessary for normal functioning of cellular units and subcellular structures. In metabolism, vitamin C functions to accept and donate hydrogen. Vitamin C also plays an important role in the synthesis of neurotransmitters, compounds which allow nerve impulse transmission between nerve axons.

Production of collagen, a protein substance in fibrous tissue, depends on ascorbic acid. Vitamin C maintains capillary integrity through the production of an intercellular cement substance. This function promotes the healing of wounds, fractures, bruises, some hemorrhages, and bleeding gums. Additionally, it reduces susceptibility to infections.

Vitamin C helps to facilitate the absorption of iron and calcium, and it is essential for the utilization of folacin.

Method of Action

Humans absorb vitamin C in the upper part of the intestine. This is accomplished by simple diffusion, a sodium-dependent active transport mechanism. When megadoses are taken (1.5 to 3.0 grams) the absorption is only 36-49% at intakes of 12 grams, only 16% is absorbed. Unabsorbed vitamin C continues into the lower bowel and causes watery stools or diarrhea.

Intakes of vitamin C above 100 milligrams do not result in an increase of ascorbic acid levels. Immediately after ingesting vitamin C, the serum level is temporarily elevated. The level subsides when the excess is picked up by the tissues that store it, or is excreted. The highest concentration of stored vitamin C is in the adrenal gland; however, the liver stores the largest amount of the vitamin in the body.

Vitamin C is excreted in the urine after enough is resorbed in the kidneys to maintain a plasma level of 1.2 to 1.4 milligrams per 100 milliliter. Vitamin C plays a vital role in the formation of collagen by catalyzing chemical changes allowing lysine and proline to bind together as collagen subunits, adding structural stability to the "complete" collagen fibers.

Vitamin C is important and necessary for the synthesis of the neurotransmitters norepinephrine and serotonin. It catalyzes the conversion of dopamine to norepinephrine and the conversion of tryptophan to serotonin.

Properties & Uses

Vitamin C has recently been the subject of controversy. Popular literature suggests large doses (one gram or more) may prevent or cure the common cold or influenza. Scientists are presently conducting studies to establish the validity of this claim. However, little scientific evidence supports the suggestion of taking large doses.

One study demonstrated vitamin C reduced severity of cold symptoms. A subsequent study indicated the result of decreasing symptoms was the result of an increased dosage at the onset of illness and was not due to an increased daily dosage during non-illness. Contradictory findings in a study of children indicated a decreased severity of symptoms with the administration of one to two grams per day. It is important to note these contradictory results may be due to differences in the vitamin C status at the beginning of the studies.

Vitamin C supplementation is beneficial for patients suffering from scurvy and its symptoms. Recommended dosages for these patients are 100 milligrams three times per day, with the alleviation of symptoms usually apparent in five days.

Consequence of Deficiency

Vitamin C deficiency is not common. Populations subject to vitamin C deficiency are alcoholics, people who consume a diet devoid of fruits and vegetables, elderly individuals on limited diets, severely ill individuals under chronic stress, and infants fed exclusively cow's milk.

Deficiency symptoms may appear the first month following deprivation, when the serum level has been reduced to 0.2 mg/dL. Scurvy is caused by severe vitamin C deficiency, with symptoms including: anemia, weakness, poor appetite and growth, tenderness to touch, swollen and inflamed gums, loosened teeth, swollen wrist and ankle joints, and shortness of breath.

Furthermore it may be observed wounds fail to heal or previous wounds break down, and infections develop easily in bleeding areas of the gum and skin. These effects are attributed to problems with collagen formation. It is also hypothesized vitamin C is involved in the blood clotting mechanism.

Psychiatric symptoms of vitamin C deficiency include depression, hysteria and hypochondriacal symptoms.

Smoking acts as an antagonist to vitamin C. Less vitamin C is available in smokers for utilization and storage, and smokers need twice the amount of vitamin C as the nonsmoker to show a comparable blood level.

Toxicity Factors

Massive doses of vitamin C have been implicated in "rebound scurvy," when the condition occurs after the cessation of administration of these massive doses. It has been hypothesized this is due to a high rate of vitamin C catabolism as an adaptation to hypersaturation. After reducing the vitamin C intake, the catabolism does not return to normal levels and this produces a vitamin C deficiency state.

Excess vitamin C excreted in the urine produces a false positive test for glucose. Excess vitamin C has also been implicated in the formation of urate and oxalate stones. However, current evidence suggests large doses (nine grams per day) produce only a small increase in urinary oxalate.

Tobacco speeds the elimination of vitamin C.

Recommended Dietary Allowance

ageRDA (mg)RNI (mg)
infants/children
0-6 months 30 25
6-12 months 35 25
1-3 years 40 30
4-10 years 45 30
males
11-14 years 50 35
15+ years 60 40
females
11-14 years 50 35
15+ years 60 40
pregnancy 70 50
lactation(1st 6 months) 95 70
(2nd 6 months) 90 70



For over thirty years, Recommended Daily Amounts has existed in the United Kingdom. It has been used to measure the adequacy of an individual's diet. However, in 1991 the Committee on Medical Aspects of Food Policy (COMA) gave forth a whole new set of figures upon the request of the Department of Health's Chief Medical Officer. Reference Nutrient Intake (RNI) is one of these sets collectively known as "Dietary Reference Values." RNI is an amount of a nutrient that is enough for almost every individuals, even someone who has high needs for the nutrient. This level of intake is, therefore, considerably higher than what most people would need. If individuals are consuming the RNI of a nutrient they are most unlikely to be deficient in that nutrient.

Food Sources

AsparagusBeet greens
BroccoliCabbage (raw)
CantaloupeCauliflower
CherriesChicory greens
Collard greensCitrus fruit
Dandelion greensGuava
Hot chili pepperMelon
Mustard greensOnion
OrangePapaya
PineapplePotato
SpinachStrawberry
TomatoTurnip greens



Abstracts

Pauling Institute

Electronic Publications

NETKE SP, NIEDZWIECKI A, ROOMI MW, TSAO CS. Ascorbic acid protects against acute aflatoxin toxicity in guinea pigs. FASEB J. (Abstracts Part I; Nos. 1-3621) 9: 141, 1995.

ROOMI MW and TSAO CS. Detoxificadon of neurotoxin P-aminopropionitrile in neurolathyrism by ascorbic acid in guinea pigs. (Abstracts Part 1) FASEB J. 8: 406, 1994

ROOMI MW and TSAO C. Influence of dietary vitamin C on thermal aggregation and aqueous phase antioxidants in scorbutic and nonscorbutic guinea pig eye lens proteins. Annual Meeting, Assoc. for Res. in Vision and Ophthalmology . (Abstract Book 36 No. 4): 2442,1995.

ROOMI MW and TSAO CS. Tissue distribution of water soluble antioxidants in scorbutic and nonscorbutic guinea pigs. (Abstracts Part II) FASEB J. 9: 5728, 1995.


1995

DUNHAM WB, TSAO C, BARTH R, and BERMAN ZS. Protection by dietary ascorbate of guinea pigs from neurolathyrism. Nutr. Res. 15(7): 993-1004, 1995.

HEMILAA H and HERMAN ZS. [Review article) Vitamin C and the common cold: a retrospective analysis of Chalmers'review. J. Am. Coll. of Nutr. 14(2): 116-123, 1995.

TSAO CS and YOUNG M. [Letter to the Editor] Molecular structure-dependent cytotoxic effect of ascorbate derivaties. In Vivo Cell Dev. Biol. 31: 87-90, 1995.


1994

HARAKEH S and JARIWALLA RJ. Comparative analysis of ascorbate and AZT effects on HIV production in persistently infected cell lines. J. Nutr. Med. 4: 393-401, 1994.

HARAKEH S, NIEDZWIECKI A and JARIWALLA RJ. Mechanistic aspects of ascorbate inhibition of human immunodeficiency virus. Chem. Biol. Interact. 91(2-3): 207-215, 1994.

JARIWALLA RJ and HARAKEH S. Ascorbic Acid and AIDS: Strategic Functions and Therapeutic Possibilities. In: Nutrition and AIDS, pp. 1 17-139. (R. Watson, Ed.). Boca Raton, FL, CRC Press, 1994.

TSAO CS, DUNHAM WB, NEYASFHTA K, and YOUNG M. Effects of ascorbic acid, sodium ascorbate, and calcium ascorbate on urine pH. Med. Sci. Res. 22: 727-728, 1994.


1993

CAMERON E and PAULING L. Cancer and Vitamin C (Updated and Expanded Edition), Philadelphia, PA: Camino Books, 278 pages, 1993.

HOFFER A and PAULING L. Hardin Jones biostatistical analysis of mortality data for a second set of cohorts of cancer patients with a large fraction surviving at the termination of the study and a comparison of survival times of cancer patients receiving large regular oral doses of vitamin C and other nutrients with similar patients not receiving those doses. J. Orthomol. Med. 8(3): 157-167, 1993.

McBEATH M and PAULING L. A case history: Lysine/ascorbate-related amelioration of angina pectoris. J. Orthomolec. Med. 8 (2): 77-78, 1993.

PAULING L. Third case report on lysine-ascorbate amelioration of angina pectoris. J. Orthomolec. Med. 8(3): 137-138, 1993.

PAULING L. Vitamin C and the common cold. J. Optimum Nutr. 6(3): 10,1993

PAULING L. [Foreword] Vitamin C: Nature's miraculous healing missile. pp. v-vi. (G Dettman, A
Kalokerinos, and I Dettman, compilers). Melbourne, Australia: Frederick Todd, 1993.

TSAO CS, MYASHITA K, LEUNG PY and YOUNG M. Cytotoxic effect of ascorbate and its derivatives on cultured malignant and nonmalignant cell lines. Anticancer Res. 13: 475-480, 1993.


1992

ALI MA, PRAKASH SS and JARIWALLA RJ. Localization of the antigenic sites and intrinsic protein kinase domain within a 300-arnino acid segment of the ribuonucleotide reductase large subunit from herpes simplex virus type 2. Virology 187: 360-367, 1992.

LONG KP, MARCUSON R, MYASHITA K and TSAO CS. Urinary excretion of calcium, dopamine, norepinephrine and epinephrine in young women following ascorbic acid ingestion. Nutr. Res. 12: 105 1 1063,1992.

PAULING L. [Book Review] Vitamin C: Its Chemistry and Biochemistry, by MB Davies, J Austin, and DA Partridge. J. Am. Chem. Soc. 114: 3171-3172, 1992.

PAULING L. Vitamin C: the key to health (speech given go the Schizophrenia Foundation). In: Stop the FDA: Save Your Health Freedom, pp. 45-62. (J Morgenthaler and S W Fowkes, Eds.) Menlo Park, CA: Health Freedom Publications, 1992.

RATH M and PAULING L. A unified theory of human cardiovascular disease leading the way to the abolition of this disease as a cause for human mortality. J. Orthomolec. Med. 7: 5-15, 1992

RATH M and PAULING L. Vitamin C and lipoprotein(a) in relation to cardiovascular disease and other diseases. J. Optim Nutrition 1: 61-64, 1992.

TSAO CS, DUNHAM WB and LEUNG PY. Effect of ascorbic acid and its derivatives on the growth of human mammary tumor xenografts in mice. CancerJ.5: 53-59,1992

YOUNG M andTSAO CS. Medium-dependent cytotoxic effect of ascorbate. J. Tiss. Cult. Meth. 14: 133-138, 1992.


1991

CAMPBELL A, JACK TC and CAMERON, E. Reticulum cell sarcoma: Two complete'spontaneous' regressions, in response to high-dose ascorbic acid therapy. Oncology 47: 495-497, 1991.

ECKERT-MAKSIC MN, ZS and MAKSIC ZB (Eds.) From Quantum Mechanics to Biochemistry: Hotwge to Linus Pauling 64: 289-575, 1991.

FLEMNG JE and BENSCH KG. Rebuttal to "Oxidative stress as a causal factor in differentiation and aging." Exp. Geront. 26: 511-515, 1991.

HARAKEH S and JARIWALLA RJ. Comparative study of the anti-H-IV activities of ascorbate and thiolcontaining reducing agents in chronically infected cells. Am. J. Clin. Nutr. 54: 123 1 S - 123 5S, 199 1.

The Pauling Catalogue: Ava Helen and Linus Pauling Papers at Oregon State University (CS Mead, J Wallace, ZS Hennan and DB MunroEds.) Corvallis, Oregon, Kerr Library Special Collections, 1991.

PAULING L. Case report: Lysine/ascorbate-related amelioration of angina pectoris. J. Orthomolec. Med. 6: 144-146,1991.

PAULING L. Effect of ascorbic acid on incidence of spontaneous mammary tumors and UV-Iight-induced skin tumors in mice. Am. J. Clin. Nutr. 54: 1252S-1255S, 1991.

PAULING L.[Letter] Respect for vitamin C. Science 254: 1712, 199 1.

PAULING L. Vitamin C and cardiovascular disease. Med. Sci. Res. 19: 398, 199 1.

PAULING L. [Letter] Vitamins and intelligence tests. Nature 353: 103, 1991.

PAULING L and RATH M. An orthmolecular theory of human health and disease. J. Orthomolec. Med. 6: 135-138,1991.

RATH M and PAULING L. Apoprotein(a) is an adhesive protein. J. Orthomolec. Med. 6: 139-143, 199 1.

RATH M and PAULING L. Solution to the puzzle of human cardiovascular disease: Its primary cause is ascorbate deficiency leading to the deposition of lipoprotein(a) and fibrinogen/fibrin in the vascular wall. J. Orthomolec. Med. 6: 125-134, 199 1.

TSAO CS and LEUNG PY. Response of human tumors to ascorbic acid and copper in the subrenal capsule assay in mice. In: Molecules in Natural Science and Medicine, pp. 493-507. (ZB) Maksic and M EckertMaksic, Eds.). Chichester, England: Ellis Horwood Ltd., 199 1.


References

Anderson, R., et al. Am J Clin Nutr., 1980; 33: 71-76.

Anonymous: Increased RDA for Vitamin C for Smokers May Still be Too Low. Family Practice News, August 1-14, 1990;20(15):29.

Anonymous: Daily Vitamin C Protects Sperm From Genetic Damage. Medical Tribune, January 16, 1992;23.

Baker, D.: Antioxidants Linked to HIV Infection. The Nutrition Report, April 1992;10(4):29/Cellular Antioxidant Status in Human Immunodeficiency Virus Replication, Nutrition Reviews, 1992;50:15-17.

Barinaga, M.: Vitamin C Gets a Little Respect: Some Researchers Say the Climate in This Controversial Field is Changing as Data Mount on the Role of Antioxidants in Disease and Health. Others Remain Skeptical. Science, October 18, 1991;254:374-376.

Beyer, K. H.: Vitamin C and Serendipity, 50 Years Ago. FASEB Journal, November 1992;6:3316-17.

Bjelke, E. Scand J Gastroenterol., 1974; 9(Suppl): 1-235.

Block, G. Am J Clin Nutr., 1991; 53: 270S-282S.

Block G.: Vitamin C, Cancer and Aging. Age, 1993;16:55-58.

Block, G.: Vitamin C and Cancer Prevention: The Epidemiologic Evidence. American Journal of Clinical Nutrition, 1991;53: 2701-282S.

Brazg, R. et al: Vitamin C Low in Diabetics, The Nutrition Report, June 1992;10(6):46/Effects of Dietary Antioxidants on LDL Oxidation in Noninsulin-Dependent Diabetics, , Clin Res, 1992;40:103A.

Bucca, C. et al: Effect of Vitamin C on Histamine Bronchial Responsiveness of Patients With Allergic Rhinitis. Annals of Allergy, October 1990;65:311-314.

Bucca, C. et al: Effect of Vitamin C on Transient Increase of Bronchoresponsiveness in Conditions Affecting the Upper Respiratory Airways. Beyond Deficiency: New Views on The Function and Health Effects of Vitamins, New York Academy of Sciences, February 9-12, 1992.

Buiatti, E. et al: A Case-Controlled Study of Gastric Cancer and Diet in Italy: II Association With Nutrients. International Journal of Cancer, 1990;45:896-901.

Calabrese, E.J. J Environ Pathol Toxicol Oncol., 1985; 5: 81-90.

Cameron, E., Pauling, L. Proc Natl Acad Sci., 1978; 75: 4538-4542.

Casaneuva, E. et al: Incidence of Premature Rupture of Membranes in Pregnant Women With Low Leukocyte Levels of Vitamin C. European Journal of Clinical Nutrition, 1991;45:401-405.

Cathcart, R. F.: Vitamin C in the Treatment of Acquired Immune Deficiency Syndrome (AIDS). Medical Hypotheses, 1984;14:423433.

Chakrabarty, S. et al: Vitamin C Deficiency Might Cause Heart Disease. The Nutrition Report, September 1992;10(9):67.

Chakrabarty, S. et al: Protective Role of Ascorbic Acid Against Lipid Peroxidation and Myocardial Injury, Molecular and Cell Biochemistry, 1992;111:41-47.

Chandra, D.B., et al. Int J Vit Nutr Res., 1986; 56: 165-168.

Chandra, R. K.: Nutrition and Immunity in the Elderly. Nutrition Reviews, December 1992;367-371.

Chen, Marianne F. et al: Effect of Ascorbic Acid on Plasma Alcohol Clearance, Journal of The American College of Nutrition, 1990;9(3):185-189.

Cheraskin, E., Ringsdorf, W.M., Jr. J Tenn Dent Assoc., 1974; 57: 177- 178.

Cheraskin, E., Ringsdorf, W.M., Jr., Medford, F.H. J Med Assoc State Alabama, 1977; 46: 39-40.

Cheraskin, E., et al. Nutr Perspect., 1978; 1: 34-36.

Cheraskin, E., et al. J Can CA., 1978; 22: 97-98.

Cheraskin, E. J Orthomol Med., 1986; 1: 241.

Cheraskin, E. Nutr Report, 1988; 6: 1, 8.

Cheraskin, E., et al. Int J Vit Nutr Res., 1973; 43: 42-55.

Cheraskin, E., Ringsdorf, W.M., Jr., Sisley, E.L. The Vitamin C Connection. Harper & Row: New York, 1983, pp. 1-279.

Cook, J.D., et al. Blood, 1984; 64: 721-726.

Cross, C. E. & Halliwell, B.: Nutrition and Human Disease: How Much Extra Vitamin C Might Smokers Need. The Lancet, April 24, 1993;341:1091.

Czajkanarin, D.M. 1984. Minerals - Food, Nutrition and Diet Therapy. M.V. Krause and L.K. Mahan. W.B. Saunders Company, Philadelphia.

Darr, D., et al: Topical Vitamin C Protects Skin. The Nutrition Report, November 1992;10(11):84.

Darr, D.: Topical Vitamin C Protects Porcine Skin From Ultraviolet Radiation-Induced Damage. British Journal of Dermatology, 1992;127:247-253.

Darr, D.: Vitamin C: Topical Skin Protector. The Nutrition Report, July 1992;10(7):49-9, 56.

Davies, S.: Suppression of Human Immunodeficiency Virus Replication by Ascorbate in Chronically and Acutely Infected Cells. Journal of Nutritional Medicine, 1990;1:345346.

Davis, R. H. et al: Vitamin C Influence on Localized Adjuvant Arthritis. Journal of The American Podiatric Medical Association, August 1990;80(8):414-418.

Dawson, E. B. et al: Effect of Ascorbic Acid Supplementation on the Sperm Quality of Smokers. Fertility and Sterility, November 1992;58(5):1034-1039.

Dorgan, J. F. & Schatzkin, A.: Antioxidant Micronutrients and Cancer Prevention. Hematology/ Oncology Clinics of North America, February 1991;5(1);43-68.

Eaton, S.B., Shostak, M., Konner, M. The Paleolithic Prescription. Harper & Row: New York, 1988, pp. 82, 130-131.

Eddy, L. et al: A Protective Role For Ascorbate in Induced Ischemic Arrest Associated with Cardiopulmonary Bypass. Journal of Applied Cardiology, 1990;5:409-414.

Emanuele, J.A. et al: Pressure Sores: How To Prevent and Treat Them. Postgraduate Medicine, May 15, 1992;91(7):113-118.

Enstrom, J. E.: Vitamin C Intake and Mortality Among a Sample of the United States Population. Epidemiology, May 1992;3(3):195-202.

Evden, F., et al. Acta Vitaminol Enzymol., 1985; 7: 131-138.

Feldman, E.B.: Ascorbic Acid Supplements and Blood Pressure -- A Four Week Pilot Study. Beyond Deficiency: New Views on the Function and Health Effects of Vitamins, New York Academy of Sciences, February 9-12, 1992;P-9.

Finglas, P.M., et al: Vitamin C Intake and Plasma Ascorbic Acid Concentration in Adolescents. The British Journal of Nutrition, 1993;69:563-576.

Flodin, N. W.: Antioxidant and Vitamin Supplementation: How Safe? The Nutrition Report, April 1992;10(4):25,32.

Fontham, E.T.H., et al. Cancer, 1988; 62: 2267-2273.

Frei, B.: Ascorbic Acid Protects Lipids in Human Plasma and Low-Density Lipoprotein Against Oxidative Damage. American Journal of Clinical Nutrition, 1991;54:1113S-18S.

Freinberg, N. & Lyte, T.: Adjunctive Ascorbic Acid Administration and Antibiotic Therapy. Journal of Dental Research, 1957;36: 260-262.

Frendenheim, J.L., Johnson, N.E., Smith, E.L. Am J Clin Nutr., 1986; 44: 863-876.

Friend, T.: Vitamin C Could Cut Cataract Risk. USA Today, Life Section, September 18, 1990.

Gaby, S.K., Singh, V.N. In: Vitamin Intake and Health: A Scientific Review.

Gaby, S.K., et al. Marcel Dekker: New York, 1991, pp. 103-161.

Garland, D. L.: Ascorbic Acid and the Eye. American Journal of Clinical Nutrition, 1991;54:1198S-1202S.

Gerster, H.: Review: Antioxidant Protection of the Ageing Macula. Age and Ageing, 1991;20:60-69.

Gey, K., et al: Poor Plasma Status of Carotene and Vitamin C is Associated With Higher Mortality From Ischemic Heart Disease and Stroke. Clinical Investigation, 1993;71:3-6.

Gey, K.F. In: Scientific Evidence For Dietary Targets in Europe. Bibliotheca Nutr Deta Vol. 37. Karger: Basil, 1986.

Ghosh, J., Das, S. Jpn J Cancer Res., 1985; 76: 1174-1178.

Ginter, E. et al. Int J Vit Nutri Res., 1977; 47: 123-134.

Goode, H. F. et al: Vitamin C Depletion and Pressure Sores in Elderly Patients With Femoral Neck Fracture. British Medical Journal, October 17, 1992;305:925-926.

Goodhart, Robert S. & Maurice E. Shills. Modern Nutrition In Health And Disease. 6th edition. Philadelphia: Lea and Febiger, 1973.

Greco, A.M. et al. Acta Vitaminol Enzymol., 1982; 4: 155-162.

Gridley, G. et al: Dietary Vitamin C Intake and Cigarette Smoking, AJPH, December 1990;81(12):1526.

Guthrie, Helen A. Introductory Nutrition. 5th edition. St. Louis: C.V. Mosby Co., 1971.

Hallberg, L., Rossander, L. Am J Clin Nutr., 1982; 35: 502-509.

Hallfrisch, J. et al: High Plasma Vitamin C Associated With Increased Plasma HDL- and HDL2-Cholesterol. American Journal of Clinical Nutrition, 1991;53:P-19.

Harakeh, S. & Jariwalla, R. J.: Comparative Study of Anti-HIV Activities of Ascorbate and Thiol-Containing Reducing Agents and Chronically HIV Infected Cells. American Journal of Clinical Nutrition, 1991;54:1231S-5S.

Harris, E. D. & Percival, S. S.: A Role For Ascorbic Acid in Copper Transport. The American Journal of Clinical Nutrition, 1991;54:1193S-7S.

Hegsted, M.D. 1976. Present Knowledge In Nutrition. 4th ed. The Nutrition Foundation pub., Washington D.C. 605.

Hemila, H.: Vitamin C and Lowering Blood Pressure: Need For Intervention Trials. Journal of Hypertension, 1991;9(11):1076-1077.

Hemila, H.: Vitamin C and Plasma Cholesterol. Critical Reviews in Food Science and Nutrition, 1992;32(1):33-57.

Henson, D. E. et al: Ascorbic Acid: Biological Functions and Relation to Cancer. Journal of The National Cancer Institute, April 17, 1991;83(8):547-550.

Henson, D. E. : Vitamin C: Biological Functions and Relations to Cancer. 1991, Summary of Abstracts.

Henson, D.E., et al: Biological Functions in Relation to Cancer, Journal of the National Cancer Institute, 1991;83:847-550.

Howe, G. R. et al: Dietary Factors and The Risk of Breast Cancer: Combined Analysis of 12 Case-Controlled Studies. Journal of The National Cancer Institute, 1990;82:561-569.

Itoh, R. et al: Serum Ascorbic Acid and HDL Cholesterol in A Healthy, Elderly Japanese Population. International Journal of Vitamin and Nutrition Research, 1990;60:360-365.

Itoh, R. et al: Sex is a Factor in The Levels of Serum Ascorbic Acid in Healthy Elderly Population. International Journal of Vitamin and Nutrition Research, 1989;59:365-372.

Jacques, P. F.: A Cross-Sectional Study of Vitamin C Intake and Blood Pressure in The Elderly. International Journal of Vitamin and Nutrition Research, 1992;62:252-255.

Jacques, P. F.: Relationship of Vitamin C Status to Cholesterol and Blood Pressure, Beyond Deficiency: New Views on the Function and Health Effects of Vitamins. New York Academy of Sciences, February 9-12, 1992;19.

Jacques, P. F.: Effect of Vitamin C on High-Density Lipoprotein Cholesterol and Blood Pressure. Journal of the American College of Nutrition, 1992;11(2):139-144.

Jacques, P.F.: Effects of Vitamin C on HDL and Blood Pressure. Journal of The American College of Nutrition, 1990;9(5):554/ Abstract 106.

Johnston, C. S. et al: Antihistamine Effects and Complications of Supplemental Vitamin C. Journal of the American Dietetic Association, August 1992;92(8):988-989.

Kennes, B., et al. Gerontology, 1983; 29: 310-310

Kirschmann, J.D. Nutrition Almanac: Nutrition Search. McGrew-Hill: New York. 1990.

Klein, M. A.: A Major Symposium on Vitamin C Sponsored by The National Cancer Institute. December 1990, pp.7. (Address: Linus Pauling Institute of Science and Medicine, 440 Page Mill Road, Palo Alto, CA 94306-2025, U.S.A. 415-327-4064)

Krause, M.V. & L.K. Mahan. 1979. Food, Nutrition and Diet Therapy. 6th ed. W.B. Saunders Company, Philadelphia. 963.

Krumdieck, C., Butterworth, C.E., Jr. Am J Clin Nutr., 1974; 27: 866- 876.

Kune, S., Kune, G.A. Watson, L.F. Nutr Cancer, 1987; 9: 21-42.

Labadie, H.: Vitamin C: From Scurvy to Ideal Vitamin Balance. La Presse Medicale, December 7, 1991;20(42):2156-2158.

Levin, E. D. et al: Clinical Trials Using Ascorbic Acid Aerosol to Aid Smoking Cessation. Drug and Alcohol Dependence, 1993;33:211-223.

Lloyd, L.E. 1978. Fundamentals of Nutrition. W.H. Freeman and Company, San Francisco, Ca.

Lohmann, W. Ann NY Acad Sci., 1987; 498: 307-311.

Lu, S-H, et al. Cancer Res., 1986; 46: 1485-1491.

Lysy, J., M.D., and Zimmerman, M.D.: Ascorbic Acid Status in Diabetes Mellitus, Nutrition Research, 1992;12:713-720.

References M - Z

MacRury, S.M. et al: Seasonal and Climatic Variation in Cholesterol and Vitamin C: Effect of Vitamin C Supplementation. Scottish Medical Journal, 1992, 37: O49 - O52. .

Manson, J. et al: Vitamin C Reduces Risk For Heart Disease. The Nutrition Report, June 1992;10(6):43.

Manson, J. et al: A Prospective Study of Vitamin C and the Incidence of Coronary Heart Disease in Women. Circulation, 1992;85:865.

Marshall, J. et al. Nutr Cancer, 1982; 3: 145-149.

Middleton, E., Jr. & Drzewiecki, G.: Effect of Ascorbic Acid and Flavonoids on Human Basophil Histamine Release. Journal of Allergy and Clinical Immunology, January 1992;89(1/Part II):278/536.

Moran, J. P. et al: Plasma Ascorbic Acid Concentrations Relate Inversely to Blood Pressure in Human Subjects. American Journal of Clinical Nutrition, 1993;57:213-7.

Murray, M.T. & Pizzorno, J.E. Encyclopedia of Natural Medicine. Rocklin, CA: Prima Publishing,1991.

Niki, E.: Action of Ascorbic Acid as a Scavenger of Active and Stable Oxygen Radicals. American Journal of Clinical Nutrition, 1991;54:1119S-24S.

O'Connor, H.J., et al. Carcinogenesis, 1985; 6: 1675-1676.

Oeffinger, K. C.: Scurvy: More Than Historical Relevance. American Family Physician, September 15, 1993;609-613.

Okunieff, P.: Interactions Between Ascorbic Acid and the Radiation of Bone Marrow, Skin and Tumor. The American Journal of Clinical Nutrition, 1991;54:1281S-3S.

Olivares, M. et al: Prevention of Iron Deficiency By Milk Fortification. ACTA Pediatr. Scand., 1989;Suppl.361:109-113.

Osilesi, O. et al: Blood Pressure and Plasma Lipids During Ascorbic Acid Supplementation in Borderline Hypertensive and Normotensive Adults. Nutrition Research, 1991;11:405-412.

Park, C. H. & Kalmer, B. F.: Growth Modulation of Human Leukemic, Preleukemic and Myeloma Progenitor Cells by L-Ascorbic Acid. American Journal of Clinical Nutrition, 1991;54:1241S-6S.

Parviainen, M. T. & Solanen, J. T.: Vitamin Status of 54-Year Old Eastern Finnish Men Throughout The Year. International Journal of Vitamin and Nutrition Research, 1990;60:47-51.

Pauling, L.: Proceedings of The National Academy of Sciences, 1974;71:4442-4446.

Pauling, L.: Proceedings of The National Academy of Sciences, 1990;87:6204-6207/9388-9890/1991.

Pauling, L.: Vitamin C and Cardiovascular Disease. Medical Sciences Research, 1991;19:399-401.

Linus Pauling: Vitamin C and the Common Cold and the Flu. The trigger in 1970 for widespread interest in vitamin C in the prevention and treatment of colds. Vitamin C is now the largest-selling vitamin in the world.

Penn, N.D. et al: The Effect of Dietary Supplementation With Vitamins A, C and E on Cell-Mediated Immune Function in Elderly Long-Stay Patients: A Randomized, Controlled Trial. Age and Ageing, 1991;20:169-174.

Pfeiffer, N.: Topical Vitamin C Limits Ultraviolet Damage to Skin. Medical Tribune, September 16, 1991;4.

Poydock, M. E.: Effect of Combined Ascorbic Acid and B-12 on Survival of Mice With Implanted Ehrlich Carcinoma and L1210Leukemia. American Journal of Clinical Nutrition, 1991;54:1261S-5S.

Ramirez, J., Flowers, N.C. Am J Clin Nutr., 1980; 33: 2079-2087.

Recommended Dietary Allowances. 1989. National Academy of Science, National Academy Press, Washington, D.C.

Retsky, K. L. et al: Ascorbic Acid Oxidation Products (S) Protect Human Low Density Lipoprotein Against Atherogenic Modification. Journal of Biological Chemistry, January 15, 1993;268(2):13041309.

Riemersma, R.A., et al. In: Vitamin E: Biochemistry and Health Implications. Vol 570. Ann NY Acad Sci: New York, 1989, pp. 291-295.

Ringsdorf, W.M., Jr., Cheraskin, E. Oral Surg., 1982; 53: 231-236.

Robertson, J., Donner, A.P., Trevithick, J.R. In: Vitamin E: Biochemistry and Health Implications. Vol 570. Ann NY Acad Sci: New York, 1989, pp. 372-382.

Robertson, J., McD.: Cataract Prevention: Time For a Clinical Trial? British Journal of Clinical Practice, November 1990; 44(11):475-476.

Robertson, J., McD.: A Possible Role for Vitamins C and E in Cataract Prevention. American Journal of Clinical Nutrition, 1991, 53: 346S - 351S.

Robinson, Corine H. 1973. Fundamentals of Normal Nutrition. The MacMillan Company, New York.

Romney, S.L., et al. Am J Obstet Gynecol., 1985; 151: 976-980.

Rose, R. & Dode, A. M.: Ocular Ascorbate Transport Metabolism. Comp. Biochem. Physiol., 1991;108(2):273-285.

Schectman, G. et al: Ascorbic Acid Requirements For Smokers: Analysis of a Population Survey. American Journal of Clinical Nutrition, 1991;53:1466-1470.

Schwartz, J. & Weiss, S.: Dietary Factors and Their Relation to Respiratory Symptoms: The Second National Health and Nutrition Examination Survey. The American Journal of Epidemiology, 1990;132(1):67-76.

Schwartz, P.L. J Am Diet Assoc., 1970; 56: 497-503.

Sergeev, A.V. B Exp Biol Med., 1984; 96: 90-92.

Segerstrale, U.: Beleaguering the Cancer Establishment. Science, book review, January 31, 1992;255:613-615.

Sies, H. et al: Oxidative Stress: From Basic Research to Clinical Application. The American Journal of Medicine, September 30, 1991;91(Suppl. 3C):3C-31S - 3C-38S.

Shilotri, P.G., Bhat, K.S. Am J Clin Nutr., 1977; 30: 1007-1081.

Simon, J. A.: Caffeine, Prostacyclin and Exercise Induced Bronchoconstriction, Chest, April 1991;99(4):1053.

Simon, J. A.: Vitamin C and Heart Disease. The Nutrition Report, August 1992;10(8):58,64.

Simon, J. A.: Vitamin C and Cardiovascular Disease: A Review. The American College of Nutrition, 1992;11(2):107-125.

Singh, V. N. & Gaby, S. K.: Premalignant Lesions: Role of Antioxidant Vitamins Beta-Carotene in Risk Reduction and Prevention of Malignant Transformation, American Journal of Clinical Nutrition, 1991;53:386S-390S.

Sowers, M.R., Wallace, R.B., Lemke, J.H. Am J Clin Nutr., 1985; 41: 1045-1053.

Stadtman, E. R.: Ascorbic Acid and Oxidative Inactivation of Proteins. American Journal of Clinical Nutrition, 1991;54:1125S-8S.

Stahelin, H. B. et al: Plasma Antioxidant Vitamins and Subsequent Cancer Mortality in Twelve-Year Follow-up of the Prospective Basel Study. American Journal of Epidemiology, 1991;133:766-75.

Stall, F. et al: Glutathione Deficiency and Human Immunodeficiency Virus Infection. The Lancet, April 11, 1992;339:909-912.

Stamler, J. In: Nutrition, Lipids and Coronary Heart Disease. Raven Press: New York, 1979, p. 25.

Strachan, D. P. et al: Ventilatory Function and Winter Fresh Fruit Consumption in a Random Sample of British Adults. Thorax, 1991;46:624-629.

Sutor, D.J., Johnston, C.S. FASEB J., 1988; 2: A851.

Tannenbaum, S.R., Wishnok, J.S., Leaf, C.D. Am J Clin Nutr., 1991; 53: 247S-250S.

Taylor, A. et al: Oxidant Stress and Cataracts: Experimental Studies, , Journal of The American College of Nutrition, October 1991;10(5):539/Abstract 21.

Thomas, P.: Vitamin C Eyed for Topical Use as A Skin Preserver: The Compound Appears to Ward Off Sun Damage and the Developer Thinks it May Also Promote Collagen Synthesis. Medical World News, March 1991;12-13.

Tolbert, L. C.: Ascorbic Acid and Autism. Autism Research Review International, 1992;6(1):4. (Department of Psychiatry and Behavioral Neurobiology, University ofAlabama at Birmingham, AL 35294. )

Trout, D.L.: Vitamin C and Cardiovascular Risk Factors. American Journal of Clinical Nutrition, 1991;53:322S-325S.

Vitale, S. et al: Plasma Antioxidants and Risk of Cortical and Nuclear Cataract, Epidemiology, May 1993;4(3):195-203.

Walji, H., Vitamin Guide: Essential nutrients for healthy living., Element: Dorset, U.K. 1992.

Walji, H., Vitamin Minerals & Dietary Supplements., Hodder Headline Plc.: London, U.K. 1994.

Wartamowicz, M., et al. Ann Nutr Metabol., 1984; 28: 186-191.

Wassertheil-Smoller, S., et al. Am J Epidemiol., 1981; 114: 714-724,

Weisburger, J. Am J Clin Nutr., 1991; 53: S226-S237.

Yoshioka, M., Matsushita, T., Chuman, Y. Int J Vit Nutr Res., 1984; 54: 343-347.

Yue, D.K. et al: Abnormalities of Ascorbic Acid Metabolism in Diabetic Control: Differences Between Diabetic Patients and Diabetic Rats, , Diabetes Research and Clinical Practice, 1990;9:239-244.

Ziemlanski, S. et al. Zy Wienie Czlowieka Metabol., 1986; 13: 7-14.